|
KMID : 0364820130490020200
|
|
Korean Journal of Microbiology
2013 Volume.49 No. 2 p.200 ~ p.204
|
|
|
Construction of Amylolytic Industrial Strains of Saccharomyces cerevisiae for Improved Ethanol Production from Raw Starch
|
|
Lim Young-Kum
Park Jin-Yeong
Lee Ja-Yeon
Choi Seung-Hyun
Chin Jong-Eon
Ko Hyun-Mi
Kim Il-Cheol
Bai Suk
|
|
|
Abstract
|
|
|
To contruct amylolytic industrial strains of Saccharomyces cerevisiae which produce ethanol efficiently from raw
starch, the Bacillus amyloliquefaciens α-amylase genes (Amy) or Aspergillus awamori glucoamylase genes (GA1)
was separately introduced into the ribosomal DNA loci in the chromosomes of the raw starch fermenting-parental
strain (ATCC 9763/YIpδAGSAδ), using double 18S rDNA-integration system. Ethanol production after 3 days of
fermentation by the strain that produced ethanol most efficiently from raw starch (ATCC 9763/YIpδAGSAδ
/YIpAG2rD) among the transformant strains was 1.5-times higher than that by the parental strain. This new strain
generated 9.2% (v/v) ethanol (72 g/L) from 20% (w/v) raw corn starch and consumed 75% of the raw starch content
during the same period.
|
|
|
KEYWORD
|
|
|
ethanol production, industrial Saccharomyces cerevisiae, raw starch
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|